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SmallAir™ : In vitro 3D human small airway epithelium

General features of SmallAir™

SmallAir is an in vitro cell model of the human small airway epithelium cultured at the air liquid interface.

We produce SmallAir™ with high standards of quality and reproducibility

  • Reconstituted using primary small airways human cells
  • Remains fully differentiated and functional for over one year in culture
  • Ready and easy to use
  • Cultured in chemically defined serum-free medium
  • Available worldwide in 24 well format

CC-10 Staining (Marker of Club Cells)

SmallAir™ is available from

Patients with several pathologies:

  • Healthy
  • COPD (Chronic Obstructive Pulmonary Disease)
  • Asthma
  • Cystic Fibrosis (Several mutations available)
  • Smoker

Schematic representation of SmallAir™

SmallAir™ characteristics

  • Air-liquid interface
  • Production of mucus
  • Active cilia-beating
  • Active ion transport
  • Tight junctions
  • Metabolic activity
  • Cytokines, chemokines, metalloproteinases release


 

Histology of SmallAir™

CC-10 Staining (Marker of Club Cells)

H/E Alcian Blue staining

MUC-5AC staining (marker of Goblet cells)

Expression of CC-10 Protein in MucilAir™ and SmallAir™

CC-10 was detected by Western Blot. As expected, SmallAir™ contained few Muc5-Ac positive cells (goblet cells). In contrast, CC-10 was not detected in MucilAir™. Instead, Muc-5Ac was highly expressed in MucilAir™.