9th - 10th November 2023 - Geneva, Switzerland
This two days hands-on training hosted by Epithelix will cover practical use of static and dynamic respiratory in vitro 3D tissues and exposure devices to evaluate acute and repeated dose inhalation toxicity and efficacy testing.
Advanced tools and immunocompetent co-culture models will be presented.
Registration fee includes the participant booklet, catering and social dinner.
Event program
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CET time |
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Thursday 9th November – Day 1 |
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08:30 |
09:00 |
Welcome introduction - Samuel Constant |
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09:00 |
09:45 |
Regulatory consideration for in vitro lung models SCAHT, Dr. Rex FitzGerald |
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09:45 |
10:15 |
Reconstructed human airway epithelium for efficacy and toxicity assessment: Fully primary human nasal, tracheal, bronchial, small-airways and alveolar 3D models EPITHELIX, Dr. Samuel Constant |
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10:15 |
10:45 |
Bioinspired lung on a chip ALVEOLIX, Dr. Janick Stucki |
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10:45 |
11:15 |
Coffee |
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11:15 |
11:45 |
Novel aerosol exposure devices for in vitro toxicity testing at the air-liquid interface VITROCELL, Dr. Paul Schumacher |
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11:45 |
12:15 |
Theoretical and practical aspects of Trans Epithelial Electrical Resistance and tissues interconnection HEPIA, Prof. Adrien Roux |
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12:15 |
12:45 |
Human airway epithelia and novel 3D immunocompetent co-culture models including macrophages and dendritic cells to study host-pathogen interactions. EPITHELIX, Dr. Samuel Constant |
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12:45 |
14:15 |
Lunch |
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14:15 |
15:45 |
HOT1 - Cell culture of 3D ALI lung models Quality criteria, maintenance, endpoints measurement. Treatment with chemicals (Part 1) – Static exposure with Liquid and Solid (Nanopress). EPITHELIX, Rosy Bonfante / Xiao-Yann Huang |
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15:45 |
16:15 |
Break |
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16:15 |
17:45 |
HOT2 - Monitoring of Cilia Motion: Cilia Beating Frequency (CBF) and Mucociliary Clearance (MCC), histology and immunostaining of ALI cultures. EPITHELIX, Guy Barbin / Nicolas Simonnet |
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19:00 |
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Social dinner at « Les Cocottes » Casino, Route d’Annecy – 74160 Saint-Julien en Genevois
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Friday 10th November – Day 2 |
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08:45 |
09:00 |
Coffee |
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09:00 |
10:30 |
HOT3 - Treatment with chemicals (Part 2) In-vitro testing of nanomaterials and pharmaceutical substances using the VITROCELL CLOUD System. VITROCELL, Dr. Paul Schumacher |
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10:30 |
11:00 |
Break |
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11:00 |
12:30 |
HOT4 - Lung-on-chip system installation and AX-12 chip handling. Initial filling and medium exchange. Assembly and software introduction. Observation of cyclic breathing motion. ALVEOLIX, Léa Todeschini |
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12:30 |
13:30 |
Lunch |
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13:30 |
15:00 |
HOT5 - Interconnection of human airway epithelia and set-ups for online TEER analysis. HEPIA, Prof. Adrien Roux / Grégoire Bouiller |
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15:00 |
15:30 |
Break |
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15:30 |
16:30 |
Wrap-up - conclusion round table discussion |
