In Vitro Evaluation of antivirals using airway tissues

Respiratory viral infections cause mild to severe diseases worldwide, such as common cold, bronchiolitis and pneumonia and are associated with huge costs for society.

Relevant human models are mandatory to test new molecules for shortening and alleviating these diseases, or to develop new therapies.

MucilAir™, SmallAir™ and AlveolAir™, holds in vitro specific mechanisms to counter invaders comparable to the in vivo situation, such as mucus production, mucociliary clearance, and secretion of defensive molecules.

A panel of clinical rhinovirus (RV), respiratory enterovirus (EV), influenza virus (Flu), respiratory syncytial virus (RSV) and coronavirus (HCoV) specimens are all capable of productive infection in MucilAir™. The virions enter and exit preferentially through the apical surface (Tapparel et al., Virology, 2013 Nov; 446:1-8). Thus, MucilAir™ is a relevant, reliable, and convenient tool for replicating respiratory viruses, including the most challenging ones.

• Evaluation of antivirals
  • Bernadett Boda, Sacha Benaoudia, Song Huang, Rosy Bonfante, Ludovic Wiszniewski, Eirini D. Tseligka, Caroline Tapparel, Samuel Constant (2020) https://www.sciencedirect.com/science/article/abs/pii/S0166354217307350
• SARS-CoV-2
  • Andrés Pizzorno, Blandine Padey, Thomas Julien, Sophie Trouillet-Assant, Aurélien Traversier, Elisabeth Errazuriz-Cerda, Julien Fouret, Julia Dubois, Alexandre Gaymard, François-Xavier Lescure, Victoria Dulière, Pauline Brun, Samuel Constant, Julien Poissy, Bruno Lina, Yazdan Yazdanpanah, Olivier Terrier, Manuel Rosa-Calatrava (2020) https://www.biorxiv.org/content/10.1101/2020.03.31.017889v1
• Rhinoviruses
  • Tapparel, C., Sobo, K., Constant, S., Huang, S., Van Belle, S., & Kaiser, L. (2013). Growth and characterization of different human rhinovirus C types in three-dimensional human airway epithelia reconstituted in vitro.Virology, 446(1), 1-8.
  • Essaidi-Laziosi M, Brito F, Benaoudia S, Royston L, Cagno V, Fernandes-Rocha M, Piuz I, Zdobnov E, Huang S, Constant S, Boldi MO, Kaiser L, Tapparel C. Propagation of respiratory viruses in human airway epithelia reveals persistent virus-specific signatures.J Allergy Clin Immunol. 2017 Aug 8.
• Influenza
  • Jazaeri Farsani, S. M., Deijs, M., Dijkman, R., Molenkamp, R., Jeeninga, R. E., Ieven, M., ... & Hoek, L. (2015). Culturing of respiratory viruses in well‐differentiated pseudostratified human airway epithelium as a tool to detect unknown viruses. Influenza and other respiratory viruses, 9(1), 51-57.
  • Elderfield, R. A., Watson, S. J., Godlee, A., Adamson, W. E., Thompson, C. I., Dunning, J., ... & Barclay, W. S. (2014). Accumulation of Human-Adapting Mutations during Circulation of A (H1N1) pdm09 Influenza Virus in Humans in the United Kingdom. Journal of virology, 88(22), 13269-13283.
  • Juozapaitis, M., Moreira, É. A., Mena, I., Giese, S., Riegger, D., Pohlmann, A., ... & Schwemmle, M. (2014). An infectious bat-derived chimeric influenza virus harbouring the entry machinery of an influenza A virus. Nature communications,5.
• Bocavirus
  • Deng, X., Li, Y., & Qiu, J. (2014). Human bocavirus 1 infects commercially available primary human airway epithelium cultures productively. Journal of virological methods, 195, 112-119.