Evaluation of permeation through respiratory epithelium

MucilAir™ and SmallAir™ allow to evaluate intranasal / bronchial / small airways permeation of drugs/xenobiotics

This assay is designed to :

Quantify and rank the permeability of compounds
Evaluate the efficiency of formulation for inhalation drug delivery

The assay is directional: from apical to basolateral, or from the basolateral to the apical compartment. The test compound is added to the apical or at the basolateral side of MucilAir™. Permeability is measured by monitoring the appearance of the test compound on the other side of the epithelium using LC-MS or ICP-MS.

Mean apparent permeability coefficients (Papp) for the test compound are compared to a reference control with known human absorption rate (Papp).

Permeability constants (Papp A->B) of 33 Reference compounds

Endpoints

Papp, Asymmetry Index, TEER values.

Molecules	Papp (cm/s) A→B	Papp (cm/s) B→A	Asymmetry Ratio
Salicilic Acid	7.7x10⁻⁵	1.7x10⁻⁵	0.2
Nicotine	2.1x10⁻⁵	3.3x10⁻⁵	1.6
Propanolol.HCl	1.2x10⁻⁵	1.6x10⁻⁵	1.3
Ibuprofen	1.1x10⁻⁵	1.9x10⁻⁵	1.7
Tripolidine.HCl	9.7x10⁻⁶	1.2x10⁻⁵	1.2
Tetracaïne	8.0x10⁻⁶	1.1x10⁻⁵	1.3
Dopamine.HCl	3.0x10⁻⁶	2.5x10⁻⁶	0.8
Atenolol	2.2x10⁻⁶	6.7x10⁻⁶	3.0

Example A->B and B->A transports and corresponding asymmetry ratio

Reference

https://www.liebertpub.com/doi/10.1089/aivt.2017.0035

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Tissues

MucilAir™ (MA) [OLD]

In vitro 3D human upper airway epithelium

Tissues

SmallAir™ (SA)

In vitro 3D human small airway epithelium